Function and application of Poem DP-95RF

SE (HLB16)

decomposition numbers among 30 test cans
C.thermoaceticum (4.6 x 10⁴ spores/can)

Structual Formula | Poem DS-100A | Poem DO-100 | Poem DP-95RF | Poem DM-100

Function and Application of Poem DP-95RF

Antibacterial

In today's Japanese market there exists a vending beverages industry. Beverages in vending machines are sold everywhere. Emulsifiers are used in these types of beverages. Emulsifier's function is generally known as emulsification, which causes an inhibitory effect of protein denaturalization. Emulsifiers are also used for their antibacterial effects. To this day, emulsifiers having an antibacterial effect is generally known as SE (Sucrose esters of fatty acids), and for the past 20 years, other forms of emulsifiers in addition to SE had not found.
Presently, we have found that DP-95RF has a better antibacterial effect than that of SE. This emulsifier's concentration is 300 to 1500ppm in coffee and 2000 to 3000ppm in corn soup and shiruko.

**MIC**
B.stearothermophilus			C.thermoaceticum			C.thermosaccharolyticum			C.thermohydrosulfuricum
ppm	DP	SE	ppm	DP	SE	ppm	DP	SE	ppm	DP	SE
0	++	++	0	++	++	0	++	++	0	++	++
4	++	++	2	++	++	10	--	+-	20	+-	+-
6	++	++	4	--	++	20	--	--	25	+-	--
8	++	++	6	--	--	50	--	--	30	+-	--
10	++	+-	8	--	--	75	--	--	35	--	--
20	--	--	10	--	--	100	--	--	40	--	--
+ growth, - growth inhibition

Bacillus stearothermophilus	Medium : bouillon	Culture condition : 55 °C, 3 days
Clostridium thermoaceticum	Medium : BR semi-liquid	Culture condition : 55 °C, 14 days
Clostridium thermosaccharolyticum	Medium : m-TGC semi-liquid	Culture condition : 55 °C, 14 days
Clostridium thermohydrosulfuricum	Medium : m-TGC semi-liquid	Culture condition : 55 °C, 14 days

This table shows the antibacterial effect of DP-95RF and SE, a test displaying minimum inhibitory concentration (MIC) by medium.
Decomposition of beverages is caused by B.stearothermophilus, C.thermoaceticum, C.thermosaccharolyticum, C.thermohydrosulfuricum, and so on.
Consequently, the antibacterial effect in B.stearothermophilus is 20ppm for both DP-95RF and SE, C.thermoaceticum is 4ppm for DP-95RF or 6ppm for SE, C.thermosacharolyticum is 10ppm for DP-95RF or 20ppm for SE, C.thermohydrosulfuricum is 35ppm for DP-95RF or 25ppm for SE.
DP-95RF has an antibacterial effect equal to SE.

Drink Formulas for Antibacterial Test

Normal Coffee		Milk Coffee (cafe au lait)
Coffee extract (Brix9)	10.6%	Coffee extract (Brix 9)	10.6%
Sugar	7.5%	Sugar	7.5%
Skim milk powder	0.8%	Fresh milk	25.0%
Whole milk powder	0.8%	Sodium bicarbonate	0.08%
Sodium bicarbonate	0.08%	Emulsifier	0.04~0.06%
Emulsifier	0.02~0.03%	Combination emulsifier	0.01%
Water		Water

Here we show data of DP-95RF's effect compared with SE on actual beverage ingredients.
These ingredients are from two types of coffee beverages.
Emulsifier's dosage is 200 to 300ppm in normal coffee and 400 to 600ppm in cafe au lait.

This is the method of antibacterial test using test tubes.

Result: Effect of emulsifier

	Normal Coffee			Cafe au lait
	emulsifier conc.(ppm)	DP-95RF	SE	emulsifier conc.(ppm)	DP-95RF	SE
B.stearothermophilus 6.3 x 10³ / ml	0	10	10	0	10	10
	200	5	10	400	10	10
	250	0	10	500	2	10
	300	0	8	600	0	10
B.stearothermophilus 1.6 x 10⁴ / ml	0	10	10	0	10	10
	200	0	9	400	10	10
	250	0	9	500	10	10
	300	0	9	600	0	10
B.coagulans 2.0 x 10⁴ / ml	0	10	10	0	10	10
	200	10	10	400	10	10
	250	9	9	500	10	10
	300	9	9	600	10	10

decomposition numbers / 10 test tubes

This table shows the decomposition numbers among 10 test tubes.
Consequently, in normal coffee the antibacterial effect of B.stearothermophilus is 200 to 250ppm for DP-95RF. In the case of cafe au lait, the antibacterial effect of B.stearothermophilus is 600ppm for DP-95RF. DP-95RF is more effective than SE in both beverages.
There is no antibacterial effect in B.coagulans for either DP-95RF or SE.

Result: Effect of emulsifier

	Normal Coffee			Cafe au lait
	emulsifier conc.(ppm)	DP-95RF	SE	emulsifier conc. (ppm)	DP-95RF	SE
C.thermoaceticum 2.3 x 10³ / ml	0	10	10	0	10	10
	200	0	9	400	0	10
	250	0	0	500	0	0
	300	0	0	600	0	0
C.thermoaceticum 4.9 x 10⁴ / ml	0	10	10	0	10	10
	200	2	9	400	1	7
	250	0	0	500	0	0
	300	0	0	600	0	0
C.thermosaccharolyticum 9.2 x 10⁴ / ml	0	10	10	0	10	10
	200	10	10	400	10	10
	250	3	10	500	10	10
	300	0	10	600	0	10
C.thermohydrosulfuricum 1.3 x 10⁴ / ml	0	10	10	0	10	10
	200	10	10	400	10	10
	250	2	10	500	10	10
	300	0	10	600	0	10

decomposition numbers / 10 test tubes

Application of DP-95RF

Normal Coffee

Formula of normal coffee	Coffee extract (Brix9)	10.6%
	Sugar	7.5%
	Skim milk powder	0.8%
	Whole milk powder	0.8%
	Sodium bicarbonate	0.08%
	Emulsifier	0.01~0.03%
	Water	-

Comparison of antibacterial function

	B.stearothermophilus 6.9 x 10³ spores / ml		C.thermoaceticum 2.4 x 10⁴ spores / ml
dosage (ppm)	Poem DP-95RF	SE (HLB16)	Poem DP-95RF	SE (HLB16)
0	26	26	30	30
100	12	12	30	30
200	1	3	6	30
300	0	2	0	0
deterioration numbers among 30 test cans

Next, we acted on the antibacterial test packed beverage into the can. The size of the beverage is 190g. The upper table shows the normal coffee ingredients. The lower table shows the test results, which gives the decomposition numbers among 30 cans. The sterilization condition is Fo=1 in the case of inoculating B.stearothermophilus and Fo=23 in the case of inoculating C.thermoaceticum.
Consequently, the antibacterial effect of B.stearothermophilus is 300ppm for DP-95RF, which is more effective than SE. The antibacterial effect of C.thermoacetium is 300ppm for both DP-95RF and SE.

Normal Milk Tea

Formula of normal milk tea	Black tea extract (Brix4.3)	7.0%
	Sugar	6.5%
	Fresh milk	10.0%
	Sodium bicarbonate	0.02%
	Emulsifier	0.03~0.05%
	Combination emulsifier	0.01%
	Water	ü

Comparison of antibacterial function

	B.stearothermophilus 5.0 x 10³ spores / ml		C.thermoaceticum 4.6 x 10⁴ spores / ml
dosage (ppm)	Poem DP-95RF	SE (HLB16)	Poem DP-95RF	SE (HLB16)
0	30	30	30	30
300	30	30	0	30
400	24	21	0	0
500	3	6	0	0
deterioration numbers among 30 test cans

In the case of normal milk tea, DP-95RF and SE have no antibacterial effect in B.stearothermophilus up to 500ppm. The antibacterial effect of C.thermoaceticum is 300ppm for both DP-95RF and SE.

Corn Soup

Formula of Corn Soup	Corn soup powder	8.5%
	Corn puree	8.0%
	Water
	Emulsifier	0.1 ~ 0.2%

Comparison of antibacterial function

This table shows the application to corn soup, changing the sterilization condition.
Consequently, the antibacterial effect of C.thermoaceticum is 2000ppm for SE in the case of only Fo=50, but is 2000ppm for DP-95RF in the case of Fo=30, Fo=40, and Fo=50.
From this result, it is possible to ease the sterilization condition by means of adding DP-95RF without affecting the corn soups flavor.